Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro.
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چکیده
منابع مشابه
Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro
Human mononuclear leukocytes were fractionated into populations of null, T and B cells by immunoabsorbent column chromatography followed by E-rosette formation and purification of T cells by differential centrifugation and osmotic lysis. The unfractionated and fractionated cell populations were first separately cultured for 14 days in plasma clots in the presence of two international units eryt...
متن کاملKidney cell lysates contain an activity that stimulates mature erythroid burst-forming-unit (mBFU-E) proliferation.
This study reports the detection of an activity that stimulates the development of a subclass of burst-forming unit-erythroid (BFU-E) progenitors giving rise to small bursts in semi-solid cultures established in the presence of saturating concentrations of erythropoietin. These progenitors are considered to be mature BFU-E. The activity is found in extracts from kidney cells and appears to be p...
متن کاملHuman burst-forming units-erythroid need direct interaction with stem cell factor for further development.
To understand the factors that regulate the early growth and development of immature erythroid progenitor cells, the burst-forming units-erythroid (BFU-E), it is necessary to have both highly purified target cells and a medium free of serum. When highly purified human blood BFU-E were cultured in a serum-free medium adequate for the growth of later erythroid progenitors, BFU-E would not grow ev...
متن کاملRegulation of human blood erythroid burst-forming unit (BFU-E) proliferation by T-lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies.
To determine whether normal T-cell subpopulations influence human blood BFU-E proliferation variably. sheep erythrocyte rosettable cells (unseparated T cells) were fractionated into subpopulations based on their differential binding to 1gM (TM cells) or lgG-coated ox erythrocytes (Ty cells). For comparative purposes. T cells. T’y cells, and T cells depleted of Ty cells (T-non-”y cells) were fur...
متن کاملpluripoietin, supports colony formation of normal human bone marrow in vitro from muitipotential (colony forming unit of granulocytes/erythrocytes/macrophages/megakaryocytes [CFU-GEMM]) and early erythroid (burst forming unit of erythroid cells [BFU-E]) progenitors, as
Colony stimulating factors (CSF) 1 a r e a class of hemopoietic regulatory glycoproteins required for survival, proliferation, and differentiation of hemopoietic stem and progenitor cells in vitro, and probably in vivo (1, 2). Many efforts have been undertaken to characterize the biochemical and biological properties of these factors. In the routine system, CSF stimulating the growth of progeni...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1978
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.147.2.324